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  Home > JCE Print > Journal of Chemical Education > Issues > 1996  > June  >
Laboratory Experiments
The Analysis of Riboflavin in Urine Using Fluorescence
Julie A. Henderleiter and Richard M. Hyslop
University of Northern Colorado, Greeley, CO 80639
Cover
June 1996
Vol. 73 No. 6
p. 563
Abstract
To become functional as scientists, chemistry students must integrate concepts learned in their classes and apply them to novel, "real life" situations. The laboratory provides an important place for the students to practice integrating concepts. This laboratory experiment, designed for undergraduate biochemistry students, requires each student to determine the amount of riboflavin excreted by his/her body following oral administration of riboflavin contained in a multi-vitamin tablet. The experimental procedure describes a protocol for the analysis of riboflavin concentration in urine using a fluorometric assay. The students must draw upon their knowledge of solution preparation, construction of a standard curve, and back-calculation procedures to determine the concentration of riboflavin in their urine. Students need to combine knowledge from general and analytical chemistry with that learned in biochemistry to complete this analysis, thus providing an opportunity to integrate knowledge while answering a novel question.
More Information
*  Citation
 Henderleiter, Julie A.; Hyslop, Richard M. J. Chem. Educ. 1996 73 563.
*  Keywords
*  History
 Created:
Last Updated:		 9/23/1999
5/22/2006
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