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1999
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In the Laboratory
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Electropolymerized Conducting Polymers as Glucose Sensors
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Omowunmi A. Sadik, Sharin Brenda, Patrick Joasil, and John Lord
SUNY at Binghamton, Department of Chemistry, P.O. Box 6016, Binghamton, NY 13902-6016
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July 1999
Vol. 76 No. 7
p. 967
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| Abstract |
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Conducting polymers are of considerable interest. Their electrochemical synthesis requires only inexpensive starting materials and low-cost equipment. This paper presents a laboratory-based experiment for possible inclusion in the undergraduate instrumental analysis laboratory curriculum. The objectives are to perform cyclic voltammetry on electropolymerized conducting polymers, to observe the effects of various parameters on the voltammogram obtained, and to perform quantitative analysis of glucose. In a typical experiment, glucose oxidase enzyme (GOx) was immobilized at an electrode surface by the electropolymerization of pyrrole from an aqueous solution containing the enzyme. The chemical activity of the immobilized GOx was evaluated by indirectly monitoring glucose oxidation using the electropolymerized PPy-modified electrode. The amount of glucose present was then determined by observing the rate at which hydrogen peroxide was produced. The magnitude of the current was linearly proportional to the concentration of glucose over the range 1 x 10-3 to 5 x 10-5M. The limit of detection was estimated at 3 times the background noise, 8 x 10-5 M glucose. The Michaelis-Menten parameters, Km and Vmax, were calculated to be approximately 1.5 x 10-3 M and 10-9 m/s, respectively, comparable with values cited in literature. This experiment illustrates the fundamental electrochemical and biosensor concepts. It reinforces the underlying principles of dynamic electrochemistry and illustrates the potential of using conducting polymers for analytical applications. The simple low-cost procedure employed should be attractive for undergraduate research projects, particularly in departments with modest means.
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| More Information |
 Citation |
Sadik, Omowunmi A.; Brenda, Sharin; Joasil, Patrick; Lord, John. J. Chem. Educ. 1999 76 967.
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Keywords |
Instrumental Methods; Electrochemistry; Enzymes; Quantitative Analysis; Redox Reactions
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History |
Created:
Last Updated: |
June 9, 1999
June 23, 2005
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| Home > JCE Print > Journal of Chemical Education > Issues >
1999
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