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1999
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In the Laboratory
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Comparison of Analytical Methods: Direct Emission versus First-Derivative Fluorometric Methods for Quinine Determination in Tonic Waters
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Siddharth Pandey, Tammie L. Borders, Carmen E. Hern‡ndez,Lindsay E. Roy, Gaddum D. Reddy,
Geo L. Martinez, Autumn Jackson, Guenevere Brown, and William E. Acree Jr.
Department of Chemistry, University of North Texas, Denton, TX 76203-0068
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January 1999
Vol. 76 No. 1
p. 85
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| Abstract |
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An undergraduate laboratory experiment is designed for the quantitative determination of quinine in tonic water samples. It is based upon direct fluorescence emission and first-derivative spectroscopic methods. Unlike other published laboratory experiments, our method exposes students to the general method of derivative spectroscopy, an important, often-used analytical technique for eliminating sample matrix and background absorbance effects and for treating overlapped spectral bands. The statistical treatment allows students to compare concentrations directly calculated from the measured fluorescence emission intensity with values obtained from the first-derivative emission spectra, to ascertain whether there is a difference between the two analytical methods. Method selection and validation are important items routinely encountered by practicing analytical chemists.
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| More Information |
 Citation |
Pandey, Siddharth; Borders, Tammie L.; Hernández, Carmen E.; Roy, Lindsay E.; Reddy, Gaddum D.; Martinez, Geo L.; Jackson, Autumn; Brown, Guenevere; Acree, William E., Jr. J. Chem. Educ. 1999 76 85.
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Keywords |
Instrumental Methods; Fluorescence Spectrometry; Laboratory Instruction; Analytical Chemistry; Statistics / Data Analysis
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History |
Created:
Last Updated: |
June 15, 1999
June 22, 2005
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| Home > JCE Print > Journal of Chemical Education > Issues >
1999
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January
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85
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