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  Home > JCE Print > Journal of Chemical Education > Issues > 1999  > September  >
In the Laboratory
A Simple Device to Demonstrate the Principles of Fluorometry
Néstor J. Delorenzi*, Cesar Araujo, Gonzalo Palazzolo, and Carlos A. Gatti
Universidad Nacional de Rosario, Facultad de Ciencias Bioquímicas y Farmacéuticas, Suipacha 531, 2000 Rosario, Argentina
Cover
September 1999
Vol. 76 No. 9
p. 1265
Abstract
A simple device to demonstrate the principles of fluorimetry was constructed using low-cost components that are readily obtained. These components were a mini ultraviolet fluorescent lamp, a plastic cell holder, borosilicate glass tubes, and a photoresistor (LDR). The LDR resistance was measured by a multimeter interfaced to a computer for data acquisition and processing. A calibration curve was constructed to relate the fluorescence of quinine bisulfate (QBS) solutions with the LDR resistance. QBS fluorescence quenching by NaCl was studied by the Stern-Volmer approach. The apparatus was designed to permit the direct observation of the following features of the fluorescence phenomenon: (i) the wavelength shift between excitation and emission light, (ii) the increase of emission intensity with increasing fluorophore concentration, and (iii) the inner filter effect for highly concentrated QBS solutions.
More Information
*  Citation
 Delorenzi, Nˇstor J.; Araujo, Cesar; Palazzolo, Gonzalo; Gatti, Carlos A. J. Chem. Educ. 1999 76 1265.
*  Keywords
 Physical Chemistry; Instrumental Methods; fluorescence*; quenching*
*  History
 Created:
Last Updated:		 July 30, 1999
June 23, 2005
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