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  Home > JCE Print > Journal of Chemical Education > Issues > 2009  > December  >
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In the Laboratory
Synthesis of Superoxide Dismutase (SOD) Enzyme Mimetics
A Bioinorganic Laboratory Experiment
Graziella Vecchio and Valeria Lanza
Dipartimento di Scienze Chimiche, Università di Catania, viale A. Doria 8 95125, Catania, Italy
Cover
December 2009
Vol. 86 No. 12
p. 1419

Abstract
The study of mimetics of metalloenzymes is a fascinating field. In the light of the clinical data surrounding the use of the superoxide dismutase enzymes, low molecular mass mimics of superoxide dismutase enzymes have recently been proposed for the treatment of a variety of diseases. We propose the synthesis of the manganese(III) complex of N,N'-bis(salicylidene)ethane-1,2-diamine ligand (H2salen)and the investigation on its superoxide dismutase ability by an indirect assay. This laboratory project was one component of a semester-long advanced undergraduate laboratory course in bioinorganic chemistry and could be expanded using several complementary techniques to characterize the ligand, the metal complex, or to investigate other activities shown by this class of complexes.
Supplement
Student handouts; Instructor note including spectra
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Citation
Vecchio, Graziella; Lanza, Valeria. J. Chem. Educ. 2009, 86, 1419.
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Keywords
Biochemistry; Bioinorganic Chemistry; Coordination Compounds; Drugs / Pharmaceuticals; Enzymes; Hands-On Learning / Manipulatives; Inorganic Chemistry; Interdisciplinary / Multidisciplinary; Laboratory Instruction; Medicinal Chemistry; Upper-Division Undergraduate; UV-Vis Spectroscopy
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History
Created:
Last Updated:
10/22/2009
10/27/2009
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Experiments, laboratory exercises, lecture demonstrations, and other descriptions of the use of chemicals, apparatus, instruments, computers, and computer interfaces are presented in the Journal of Chemical Education as illustrative of new or improved ideas or concepts in chemistry instruction and are directed at qualified teachers. Although every effort is made to assure and encourage safe practices and safe use of chemicals, the Journal of Chemical Education cannot assume responsibility for uses made of its published materials. Many chemicals are hazardous. Precautions for the safe use of hazardous chemicals and directions for their proper disposal are described in the Material Safety Data Sheets and on the labels. We strongly urge all those planning to use materials from our pages to make choices and to develop procedures for laboratory and classroom safety in accordance with local needs and situations.
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